By Herbert Begemann
-Enhanced sixth version includes extra illustrations and documented figures
-A excellent reference for hematological and oncological departments and the clincal labortory
-Classified findings from the area future health Organization
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Extra resources for Atlas of Clinical Hematology
The basophilic myeloblasts differentiate into promyelocytes by the incorporation of coarse granules. Although some promyelocytes display eosinophilic or basophilic features in their cytoplasmic granulations, the cells generally are considered to be uncommitted at this stage. Subsequently, in the myelocyte stage, these differences become very pronounced. Wright's theory that platelets are fragments from the cytoplasm of megakaryocytes is now generally accepted. The origin of monocytes has been widely discussed in recent years.
This solution hemolyzes and stains simultaneously. Stain for 40 min. When staining is completed, wash the slide carefully by dipping in tap water. Allow to dry in a vertical position. A thin blood smear should additionally be prepared so that the parasites can be accurately identified if doubt exists. Often this is difficult in thick smears. Thick smear preparations for trypanosomes (T. gambiense, T. rhodesiense, T. cruzi) are stained in the same fashion as for malaria parasites. The same thick smear method is used to examine for Borrelia recurrentis.
4. Rinse briefly, air dry. 5. Nuclear stain with hemalum (see p. t 2), 8-10 mm. 6. Rinse, air dry. Mount in glycerin jelly if oil-immersion examination is planned. Result. One hundred (or a multiple of 100) band and segmented neutrophils are counted, and each cell is assigned a score of 0-5 depending on the intensity of the reaction. The percentage of cells assigned to a specific reaction class is multiplied by the corresponding class factor (see Table 2) to yield the LAP score of the smear. Table 2 Reaction class Neutrophils Multiplication factor Phosphatase activity 0 1 2 3 4 5 23% 25% 32% 17% 3% 0% 0 1 2 3 4 5 0 25 64 51 12 0 Phosphatase score 18 152 Interpretation.