Blood Cells: A Practical Guide by Barbara J. Bain

By Barbara J. Bain

  • Enables either the haematologist and laboratory scientist to spot blood mobile gains, from the commonest to the extra obscure
  • Provides crucial info on tools of assortment, blood movie training and marking, including the foundations of guide and automatic blood counts
  • Completely revised and up to date, incorporating a lot newly released details: now contains suggestion on additional exams whilst a selected analysis is suspected
  • 400 top of the range images to assist with blood telephone identification
  • Highlights the aim and scientific relevance of haematology laboratory exams throughout

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Example text

Conversion is almost instantaneous and methaemoglobin, but not sulphaemoglobin, is converted. This method correlates well with the reference method that is employed for calibration. This method is suitable for use with a spectrophotometer and has also been incorporated into several automated instruments. Hb can also be measured following conversion to azidmethaemoglobin by the addition of sodium nitrate and sodium azide. ), which employs measurements at two wavelengths, 570 and 880 nm, to permit compensation for turbidity.

Various methods of tracking over a slide have been proposed to attempt to overcome errors due to maldistribution (Fig. 3). The method shown in Fig. 3a compensates for maldistribution between the body and the tail, but not for maldistribution between the centre and the edge, whereas the ‘battlement’ method shown in Fig. 3b tends to do the reverse, since the customary 100‐cell differential count will not cover a very large proportion of the length of the blood film. A modified battlement track (Fig.

Particles can be counted and sized either by electrical impedance or by light scattering. Automated instruments have at least two channels. In one channel a diluent is added and red cells are counted and sized. In another channel a lytic agent is added, together with diluent, to reduce red cells to stroma, leaving the white cells intact for counting and also producing a solution in which Hb can be measured. g. impedance technology with current of various frequencies, light 30 Chapter 2 scattering and light absorbance.

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